7QSH
23S ribosomal RNA Sarcin Ricin Loop 27-nt fragment containing a Xanthosine residue at position 2648
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-18 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9 |
Spacegroup name | P 43 |
Unit cell lengths | 29.500, 29.500, 76.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.500 - 0.860 |
R-factor | 0.1476 |
Rwork | 0.147 |
R-free | 0.16630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dvz |
RMSD bond length | 0.005 |
RMSD bond angle | 1.421 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 0.880 |
High resolution limit [Å] | 0.860 | 0.860 |
Number of reflections | 54707 | 3549 |
<I/σ(I)> | 56 | |
Completeness [%] | 98.9 | |
Redundancy | 20 | |
CC(1/2) | 1.000 | 0.049 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Ammonium sulfate, magnesium acetate, MES pH5.6 |