7QSH

23S ribosomal RNA Sarcin Ricin Loop 27-nt fragment containing a Xanthosine residue at position 2648

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-18
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9
Spacegroup name	P 43
Unit cell lengths	29.500, 29.500, 76.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.500 - 0.860
R-factor	0.1476
Rwork	0.147
R-free	0.16630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dvz
RMSD bond length	0.005
RMSD bond angle	1.421
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	0.880
High resolution limit [Å]	0.860	0.860
Number of reflections	54707	3549
<I/σ(I)>	56
Completeness [%]	98.9
Redundancy	20
CC(1/2)	1.000	0.049

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Ammonium sulfate, magnesium acetate, MES pH5.6