7QSG

Methylmannose polysaccharide mannosyltransferase from M. hassiacum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03320
Spacegroup name	C 1 2 1
Unit cell lengths	136.261, 77.400, 101.210
Unit cell angles	90.00, 106.49, 90.00

Refinement procedure

Resolution	66.590 - 2.750
R-factor	0.2702
Rwork	0.269
R-free	0.28670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4n9w
RMSD bond length	0.002
RMSD bond angle	0.468
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.590	2.848
High resolution limit [Å]	2.750	2.750
Rmerge	0.034	0.577
Rmeas	0.048	0.816
Rpim	0.034	0.577
Number of reflections	26401	2609
<I/σ(I)>	9.97	1.42
Completeness [%]	99.6
Redundancy	1.9
CC(1/2)	0.999	0.649

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M Na-HEPES pH 7.5, 0.2 M sodium acetate, 25% (w/v) PEG 3350