7QS4

Crystal structure of B30.2 PRYSPRY domain of TRIM36

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-05
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.00002
Spacegroup name	P 21 21 21
Unit cell lengths	91.736, 93.064, 103.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.530 - 2.250
R-factor	0.2049
Rwork	0.203
R-free	0.24490
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.248
Data reduction software	XDS
Data scaling software	Aimless (0.7.7)
Phasing software	SHARP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.530	46.530	2.330
High resolution limit [Å]	2.250	8.710	2.250
Rmerge	0.063	0.034	0.927
Rmeas	0.070	0.036	1.040
Rpim	0.022	0.012	0.335
Number of reflections	42900	840	4139
<I/σ(I)>	16.9		2
Completeness [%]	100.0	99.6	100
Redundancy	9.3	8.5	9.4
CC(1/2)	0.999	0.999	0.861

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	24% PEG 3350, 0.15M sodium citrate