7QS4
Crystal structure of B30.2 PRYSPRY domain of TRIM36
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-05 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1.00002 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 91.736, 93.064, 103.850 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.530 - 2.250 |
R-factor | 0.2049 |
Rwork | 0.203 |
R-free | 0.24490 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.248 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.7) |
Phasing software | SHARP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.530 | 46.530 | 2.330 |
High resolution limit [Å] | 2.250 | 8.710 | 2.250 |
Rmerge | 0.063 | 0.034 | 0.927 |
Rmeas | 0.070 | 0.036 | 1.040 |
Rpim | 0.022 | 0.012 | 0.335 |
Number of reflections | 42900 | 840 | 4139 |
<I/σ(I)> | 16.9 | 2 | |
Completeness [%] | 100.0 | 99.6 | 100 |
Redundancy | 9.3 | 8.5 | 9.4 |
CC(1/2) | 0.999 | 0.999 | 0.861 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 24% PEG 3350, 0.15M sodium citrate |