7QRT
Structural insight into the Scribble PDZ domains interaction with the oncogenic Human T-cell lymphotrophic virus-1 (HTLV-1) Tax1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 100.757, 100.757, 68.525 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 53.890 - 1.900 |
| R-factor | 0.215 |
| Rwork | 0.213 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vwk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.155 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER (1.15.2-3472-000) |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.890 | 1.910 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Number of reflections | 17472 | 1634 |
| <I/σ(I)> | 6.1 | |
| Completeness [%] | 99.0 | |
| Redundancy | 4.4 | |
| CC(1/2) | 0.990 | 0.250 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 2.0M Ammonium sulfate, 20% w/v PEG 3350 |
