7QRS

Structural insight into the Scribble PDZ domains interaction with the oncogenic Human T-cell lymphotrophic virus-1 (HTLV-1) Tax1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 65
Unit cell lengths	50.257, 50.257, 184.398
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.140 - 1.770
R-factor	0.2426
Rwork	0.241
R-free	0.27550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vwc
RMSD bond length	0.009
RMSD bond angle	0.975
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER (1.15.2-3472-000)
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.100	1.800
High resolution limit [Å]	1.770	1.770
Rmerge	0.056	0.760
Number of reflections	25599	2557
<I/σ(I)>	16.48
Completeness [%]	99.3
Redundancy	2.7
CC(1/2)	0.990	0.430

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1M Zinc chloride, 0.1M Sodium acetate ph5, 20% w/v PEG 1500