7QRN
Crystal structure of Ovalbumin-related protein X (OVAX) complexed with fondaparinux
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.504, 68.069, 108.472 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.090 - 1.830 |
| R-factor | 0.179 |
| Rwork | 0.177 |
| R-free | 0.21250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ova |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.660 | 1.863 |
| High resolution limit [Å] | 1.830 | 1.832 |
| Rmerge | 0.044 | 0.790 |
| Rmeas | 0.048 | 0.883 |
| Rpim | 0.020 | 0.385 |
| Number of reflections | 35271 | 1691 |
| <I/σ(I)> | 17.9 | 1.6 |
| Completeness [%] | 99.1 | 97.9 |
| Redundancy | 5.7 | 5 |
| CC(1/2) | 1.000 | 0.801 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | phosphate/citrate, LiSO4, PEG 1500 |
