7QRL
LytM domain of DipM, a coordinator of a complex net of autolysins in Caulobacter crescentus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979185 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 65.869, 105.839, 108.431 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.750 - 2.250 |
| R-factor | 0.1892 |
| Rwork | 0.188 |
| R-free | 0.21910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5kvp |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.569 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.750 | 2.320 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rpim | 0.102 | 0.474 |
| Number of reflections | 36758 | 3319 |
| <I/σ(I)> | 8 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.1 | 11.1 |
| CC(1/2) | 0.990 | 0.703 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 291 | 0.02M Magnesium chloride, 0.1M Hepes pH 7.5 and 22% Polyacrylic acid sodium salt 5100 . |
