7QRJ
Crystal structure of Zamilon vitis protein Zav_19
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 154.367, 103.258, 102.299 |
Unit cell angles | 90.00, 131.48, 90.00 |
Refinement procedure
Resolution | 45.250 - 1.380 |
R-factor | 0.142 |
Rwork | 0.141 |
R-free | 0.16500 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.1) |
Phasing software | SHELX |
Refinement software | PHENIX (1.19.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.250 | 45.250 | 1.400 |
High resolution limit [Å] | 1.380 | 7.560 | 1.380 |
Rmerge | 0.057 | 0.059 | 0.220 |
Rmeas | 0.064 | 0.067 | 0.255 |
Rpim | 0.029 | 0.032 | 0.127 |
Total number of observations | 1177549 | 7350 | 47557 |
Number of reflections | 245617 | 1564 | 12112 |
<I/σ(I)> | 13.7 | 25.7 | 4.1 |
Completeness [%] | 99.9 | 99.7 | 99.4 |
Redundancy | 4.8 | 4.7 | 3.9 |
CC(1/2) | 0.994 | 0.966 | 0.937 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | PEG 3350, ammonium citrate |