7QR4

human CPEB3 HDV-like ribozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	110
Detector technology	PIXEL
Collection date	2018-07-05
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00004
Spacegroup name	C 2 2 21
Unit cell lengths	79.451, 131.879, 90.456
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.940 - 2.830
R-factor	0.2223
Rwork	0.220
R-free	0.26760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1drz
RMSD bond length	0.003
RMSD bond angle	0.615
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.055	3.124
High resolution limit [Å]	2.825	2.825
Number of reflections	6828	342
<I/σ(I)>	16.7
Completeness [%]	92.6
Redundancy	12.8
CC(1/2)	0.998	0.574

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	20-24% PEG 3350, 0.2 M sodium citrate tribasic dihydrate and 30-200 mM HEPES-KOH pH 7.5