7QR2

Crystal structure of chicken Ovalbumin-related protein X

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97857
Spacegroup name	P 21 21 21
Unit cell lengths	53.742, 64.275, 115.525
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.030 - 1.640
R-factor	0.178
Rwork	0.176
R-free	0.20440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ova
RMSD bond length	0.010
RMSD bond angle	1.055
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.167	1.670
High resolution limit [Å]	1.640	1.640
Rmerge	0.051	0.674
Rmeas	0.056	0.731
Rpim	0.022	0.279
Number of reflections	49372	2417
<I/σ(I)>	17.5	2.3
Completeness [%]	99.5	99.3
Redundancy	6.6	6.7
CC(1/2)	0.999	0.894

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	293	phosphate/citrate buffer, LiSO4, PEG1500