7QQV
SpCas9 bound to FANCF off-target3 DNA substrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-12 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 179.019, 67.145, 187.767 |
Unit cell angles | 90.00, 111.14, 90.00 |
Refinement procedure
Resolution | 47.810 - 2.250 |
R-factor | 0.1965 |
Rwork | 0.195 |
R-free | 0.23040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fq5 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.612 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.230 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.120 | 1.710 |
Number of reflections | 98463 | 9812 |
<I/σ(I)> | 11.4 | 1 |
Completeness [%] | 99.2 | 99 |
Redundancy | 6.9 | 7.1 |
CC(1/2) | 0.998 | 0.497 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 0.1 M Tris-acetate pH 8.5, 0.3-0.5 M KSCN, 17-19% PEG3350 |