7QQS

SpCas9 bound to FANCF on-target DNA substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-07
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	177.906, 67.055, 187.397
Unit cell angles	90.00, 111.11, 90.00

Refinement procedure

Resolution	47.720 - 2.400
R-factor	0.1987
Rwork	0.197
R-free	0.22880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fq5
RMSD bond length	0.003
RMSD bond angle	0.609
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.720	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.120	1.590
Number of reflections	81059	8039
<I/σ(I)>	13.9	1.18
Completeness [%]	99.9	99.9
Redundancy	6.8	6.8
CC(1/2)	0.998	0.487

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293.15	0.1 M Tris-acetate pH 8.5, 0.3-0.5 M KSCN, 17-19% PEG3350