7QQ8
Structure of E.coli Class 2 L-asparaginase EcAIII, mutant RDM1-8 (G206Y, R207Q, D210P, S211T)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | agilent |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-24 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.5406 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.005, 74.885, 147.835 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.161 - 1.800 |
| Rwork | 0.196 |
| R-free | 0.23650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zal |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.609 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.161 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.076 | 0.519 |
| Rmeas | 0.092 | 0.631 |
| Number of reflections | 48975 | 2945 |
| <I/σ(I)> | 8 | 1.6 |
| Completeness [%] | 93.6 | |
| Redundancy | 2.6 | |
| CC(1/2) | 0.996 | 0.692 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20% PEG 4000; 15% PEG 400; 0,2 M MgCl2 in 0.1M Tris-HCl pH 8.5 |
