7QQ8
Structure of E.coli Class 2 L-asparaginase EcAIII, mutant RDM1-8 (G206Y, R207Q, D210P, S211T)
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | agilent |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-24 |
Detector | RIGAKU HyPix-6000HE |
Wavelength(s) | 1.5406 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.005, 74.885, 147.835 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.161 - 1.800 |
Rwork | 0.196 |
R-free | 0.23650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zal |
RMSD bond length | 0.011 |
RMSD bond angle | 1.609 |
Data reduction software | CrysalisPro |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.161 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.076 | 0.519 |
Rmeas | 0.092 | 0.631 |
Number of reflections | 48975 | 2945 |
<I/σ(I)> | 8 | 1.6 |
Completeness [%] | 93.6 | |
Redundancy | 2.6 | |
CC(1/2) | 0.996 | 0.692 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20% PEG 4000; 15% PEG 400; 0,2 M MgCl2 in 0.1M Tris-HCl pH 8.5 |