7QQ8

Structure of E.coli Class 2 L-asparaginase EcAIII, mutant RDM1-8 (G206Y, R207Q, D210P, S211T)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	agilent
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-24
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.5406
Spacegroup name	P 21 21 21
Unit cell lengths	50.005, 74.885, 147.835
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.161 - 1.800
Rwork	0.196
R-free	0.23650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zal
RMSD bond length	0.011
RMSD bond angle	1.609
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.161	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.076	0.519
Rmeas	0.092	0.631
Number of reflections	48975	2945
<I/σ(I)>	8	1.6
Completeness [%]	93.6
Redundancy	2.6
CC(1/2)	0.996	0.692

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	20% PEG 4000; 15% PEG 400; 0,2 M MgCl2 in 0.1M Tris-HCl pH 8.5