7QPX
Complex of rice blast (Magnaporthe oryzae) effector protein AVR-PikC with an engineered HMA domain of Pikp-1 (Pikp-SNK-EKE) from rice (Oryza sativa)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.353, 83.130, 107.035 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.710 - 2.050 |
R-factor | 0.2056 |
Rwork | 0.204 |
R-free | 0.22660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7a8w |
RMSD bond length | 0.008 |
RMSD bond angle | 1.519 |
Data reduction software | XDS |
Data scaling software | Aimless (0.6.1) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.710 | 46.710 | 2.110 |
High resolution limit [Å] | 2.050 | 8.940 | 2.050 |
Rmerge | 0.081 | 0.038 | 1.048 |
Rmeas | 0.085 | 0.041 | 1.103 |
Rpim | 0.027 | 0.014 | 0.340 |
Number of reflections | 37795 | 534 | 2886 |
<I/σ(I)> | 15.2 | ||
Completeness [%] | 99.8 | 99.2 | 99.4 |
Redundancy | 9.9 | 8 | 10.1 |
CC(1/2) | 0.999 | 0.998 | 0.838 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | NULL |