7QOX
Factor XI and Plasma Kallikrein apple domain structures reveals different kininogen bound complexes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2015-05-25 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.976 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.543, 68.258, 120.311 |
Unit cell angles | 90.00, 96.55, 90.00 |
Refinement procedure
Resolution | 29.640 - 2.320 |
R-factor | 0.2019 |
Rwork | 0.199 |
R-free | 0.25260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6i58 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.640 | 2.400 |
High resolution limit [Å] | 2.320 | 2.320 |
Number of reflections | 38760 | 9002 |
<I/σ(I)> | 7.6 | |
Completeness [%] | 99.4 | |
Redundancy | 3.4 | |
CC(1/2) | 0.987 | 0.597 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 288 | Morpheus screen H9 condition: 0.1M amino acids, 0.1M buffer system 3 pH8.5, 30% precipitant mix 1. |