7QNQ
Structure of the Aux2 relaxosome protein of plasmid pLS20
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 137.854, 137.854, 121.709 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 61.650 - 1.890 |
R-factor | 0.1814 |
Rwork | 0.180 |
R-free | 0.20100 |
Structure solution method | AB INITIO PHASING |
RMSD bond length | 0.005 |
RMSD bond angle | 0.662 |
Data reduction software | autoPROC (1.0.5) |
Data scaling software | Aimless (0.7.4) |
Phasing software | Arcimboldo |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 97.480 | 1.960 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmerge | 0.190 | 3.150 |
Rmeas | 0.200 | 3.100 |
Number of reflections | 87560 | 4373 |
<I/σ(I)> | 14.3 | 1.5 |
Completeness [%] | 96.1 | 57.4 |
Redundancy | 26.5 | 26.5 |
CC(1/2) | 0.998 | 0.522 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | COUNTER-DIFFUSION | 300 | 0.06 M D-Glucose, 0.06 M D-Mannose, 0.06 M D-Galactose, 0.06 M L-Fucose, 0.06 M D-Xylose, 0.06 M N-Acetyl-D-Glucosamine, 0.06 M Tris-Bicine pH8.5, 20% v/v Ethylene glycol, 10 % w/v PEG 8000 |