7QNP

Designed Armadillo repeat protein N(A4)M4C(AII) co-crystallized with hen egg white lysozyme

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	56.592, 62.655, 108.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.997 - 1.586
Rwork	0.186
R-free	0.21850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5aei
RMSD bond length	0.018
RMSD bond angle	2.170
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.000	1.650
High resolution limit [Å]	1.586	1.586
Rmerge	0.056
Rmeas	0.059
Rpim	0.016	0.384
Number of reflections	53083	5191
<I/σ(I)>	22.36	1.48
Completeness [%]	100.0	99.98
Redundancy	13.4	13.8
CC(1/2)	1.000	0.702

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	35% (v/v) dioxane, pH undefined