7QNM
Crystallization and structural analyses of ZgHAD, a L-2-haloacid dehalogenase from the marine Flavobacterium Zobellia galactanivorans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-15 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.980114 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 76.165, 132.789, 275.698 |
Unit cell angles | 90.00, 92.31, 90.00 |
Refinement procedure
Resolution | 49.510 - 2.730 |
R-factor | 0.20881 |
Rwork | 0.206 |
R-free | 0.25434 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7asz |
RMSD bond length | 0.009 |
RMSD bond angle | 1.502 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.510 | 2.800 |
High resolution limit [Å] | 2.730 | 2.750 |
Rmeas | 0.143 | 1.475 |
Rpim | 0.054 | 0.780 |
Number of reflections | 144327 | 36555 |
<I/σ(I)> | 5 | 1 |
Completeness [%] | 99.1 | 83.7 |
Redundancy | 6.9 | 6.2 |
CC(1/2) | 0.998 | 0.517 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 25% (w/v) PEG3350, 0.1 M Tris-HCl pH 8.5 and 0.2 M NaCl |