7QNL

LOV2-DARPIN FUSION - D4_DeltaLOV

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 42 21 2
Unit cell lengths	89.741, 89.741, 69.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.456 - 1.710
Rwork	0.197
R-free	0.26350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1svx
RMSD bond length	0.013
RMSD bond angle	1.752
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.460	1.850
High resolution limit [Å]	1.710	1.710
Rmerge	0.066
Rpim	0.013
Number of reflections	22744	1138
<I/σ(I)>	28	1.6
Completeness [%]	95.8
Redundancy	25.2
CC(1/2)	0.999	0.584

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2 M ammonium sulphate, 2% PEG400, 100 mM HEPES, pH 7.5