7QNG

Structure of a MHC I-Tapasin-ERp57 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033
Spacegroup name	P 2 21 21
Unit cell lengths	74.760, 168.530, 187.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.000 - 2.700
R-factor	0.1999
Rwork	0.198
R-free	0.22890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6eny
RMSD bond length	0.009
RMSD bond angle	1.357
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PDB-REDO

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	2.797
High resolution limit [Å]	2.700	2.700
Number of reflections	65719	6491
<I/σ(I)>	14.9
Completeness [%]	99.8
Redundancy	5.9
CC(1/2)	0.998	0.443

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	100 mM Gly-Gly, AMPD, pH 8.5, 300 mM lithium sulfate, 300 mM sodium sulfate, 300 mM potassium sulfate, 20% (v/v) PEG8000, 40% (v/v) 1,5-pentanediol