7QLH

Crystal structure of S-layer protein SlpA from Lactobacillus amylovorus, domain I (aa 48-213)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.94290
Spacegroup name	P 21 21 2
Unit cell lengths	49.543, 91.756, 93.616
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.790 - 2.300
R-factor	0.2262
Rwork	0.223
R-free	0.28310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7qle
RMSD bond length	0.003
RMSD bond angle	0.532
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.790	2.382
High resolution limit [Å]	2.300	2.300
Rmerge	0.057	0.473
Number of reflections	19468	1908
<I/σ(I)>	19.32	4.17
Completeness [%]	99.0
Redundancy	6.3
CC(1/2)	0.999	0.959

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	Crystallization condition 0.1 M MES, 40 % v/v MPD, 5 % w/v PEG 8000 Protein solution 20 mg/ml in 20 mM HEPES pH 8.0, 100 mM NaCl Setup 0.5 ul crystallization condition + 0.5 ul protein solution