7QJI

X-Ray Structure of apo-EleNRMT in complex with two Nanobodies at 4.1A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-01-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.999869
Spacegroup name	P 1 21 1
Unit cell lengths	93.270, 122.250, 149.110
Unit cell angles	90.00, 107.79, 90.00

Refinement procedure

Resolution	11.990 - 4.100
R-factor	0.2693
Rwork	0.267
R-free	0.31550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDBID 7QIA
RMSD bond length	0.003
RMSD bond angle	0.603
Data reduction software	XDS (Version Feb5 2021)
Data scaling software	XSCALE (Version Feb5 2021)
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	12.000	4.200
High resolution limit [Å]	4.100	4.100
Rmeas	0.112	1.536
Number of reflections	25065	1742
<I/σ(I)>	15.36	1.81
Completeness [%]	99.2	98.9
Redundancy	28.2	27.1
CC(1/2)	0.999	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	50 mM MgAc, 50 mM HEPES pH 7.2-7.6 and 25-30% PEG400