7QIJ

Complex of the Yersinia enterocolitica Type III secretion export gate YscV with substrate:chaperone complex YscX:YscY

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	143.460, 324.920, 369.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.830 - 4.100
R-factor	0.3064
Rwork	0.305
R-free	0.32480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7alw
RMSD bond length	0.002
RMSD bond angle	0.604
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.830	3.920
High resolution limit [Å]	3.700	3.700
Rmeas	0.170	2.893
Number of reflections	135378	28922
<I/σ(I)>	7.1	0.65
Completeness [%]	99.5	98.1
Redundancy	5.2	5.23
CC(1/2)	0.998	0.395

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Protein Concentration: 3-5 mg/ml; Protein Buffer: 20 mM Tris pH 8.0, 150 mM NaCl, 5 mM TCEP; Reservoir Solution: 0.1 M Hepes pH 6.8-8.0, 1 % PEG 2'000 MME, 0.8-1.2 M sodium succinate; Drop Ratio: 0.33 uL Reservoir + 0.66 uL Protein