7QII
Complex of the Yersinia enterocolitica Type III secretion proteins YscX and YscY
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 179.268, 179.268, 41.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.820 - 3.290 |
R-factor | 0.2296 |
Rwork | 0.224 |
R-free | 0.28310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | D_1292118683 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.766 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.490 |
High resolution limit [Å] | 3.290 | 3.290 |
Rmeas | 0.130 | 1.255 |
Number of reflections | 5368 | 839 |
<I/σ(I)> | 14.55 | 2.25 |
Completeness [%] | 99.8 | 99.3 |
Redundancy | 12.7 | 13 |
CC(1/2) | 0.999 | 0.773 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Protein Concentration: 5 mg/mL; Protein Buffer: 10 mM Tris pH 8.0, 50 mM NaCl, 5 % (w/v) sucrose, 2.5 mM TCEP; Reservoir Solution: 0.1 M Sodium succinate pH 6-6.5, 4.2-4.4 M NaCl, 4 % acetonitrile; Drop Ratio: 0.5 - 1 uL Reservoir + 1 uL Protein |