7QI9
Crystal structure of Mycobacterium hassiacum glucosyl-3-phosphoglycerate synthase at pH 7.2 in complex with UDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96112 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.619, 90.415, 95.509 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.400 - 1.230 |
| R-factor | 0.1429 |
| Rwork | 0.142 |
| R-free | 0.16180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7qpc |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.940 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.270 |
| High resolution limit [Å] | 1.230 | 1.230 |
| Rmerge | 0.040 | 0.554 |
| Rmeas | 0.052 | 0.716 |
| Rpim | 0.024 | 0.357 |
| Number of reflections | 171306 | 13931 |
| <I/σ(I)> | 15.1 | 1.8 |
| Completeness [%] | 95.6 | 80.5 |
| Redundancy | 4.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 298 | 0.2M Potassium Sodium Tartrate pH 7.2, 20% PEG 3350 |
