7QI9

Crystal structure of Mycobacterium hassiacum glucosyl-3-phosphoglycerate synthase at pH 7.2 in complex with UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.96112
Spacegroup name	P 21 21 21
Unit cell lengths	71.619, 90.415, 95.509
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.400 - 1.230
R-factor	0.1429
Rwork	0.142
R-free	0.16180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7qpc
RMSD bond length	0.007
RMSD bond angle	0.940
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.270
High resolution limit [Å]	1.230	1.230
Rmerge	0.040	0.554
Rmeas	0.052	0.716
Rpim	0.024	0.357
Number of reflections	171306	13931
<I/σ(I)>	15.1	1.8
Completeness [%]	95.6	80.5
Redundancy	4.6	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	298	0.2M Potassium Sodium Tartrate pH 7.2, 20% PEG 3350