7QHY

Structure of a Kluyveromyces lactis protein involved in RNA decay

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97856
Spacegroup name	P 32 2 1
Unit cell lengths	76.820, 76.820, 174.310
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.760 - 2.450
R-factor	0.2314
Rwork	0.230
R-free	0.26870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold model
RMSD bond length	0.008
RMSD bond angle	0.970
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.770	2.510
High resolution limit [Å]	2.450	2.450
Rmerge	0.011	3.897
Number of reflections	22619	1648
<I/σ(I)>	30.8	1.1
Completeness [%]	99.8	100
Redundancy	59.8
CC(1/2)	0.999	0.605

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		280	0.1 M sodium citrate pH 5.6, 0.9-1 M Li2SO4 and 0.6 M ammonium sulfate