7QGJ
Apo structure of BIR2 Domain of BIRC2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.999998 |
| Spacegroup name | H 3 |
| Unit cell lengths | 87.241, 87.241, 59.625 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.810 - 1.300 |
| R-factor | 0.1431 |
| Rwork | 0.142 |
| R-free | 0.15520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AF-Q13490-F1 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.427 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.810 | 46.810 | 1.320 |
| High resolution limit [Å] | 1.300 | 7.000 | 1.300 |
| Rmerge | 0.070 | 0.093 | 0.849 |
| Rmeas | 0.080 | 0.114 | 0.973 |
| Rpim | 0.038 | 0.063 | 0.464 |
| Total number of observations | 206199 | 550 | 8898 |
| Number of reflections | 41245 | 213 | 2119 |
| <I/σ(I)> | 13.1 | 22.4 | 1.8 |
| Completeness [%] | 99.0 | 79.5 | 98 |
| Redundancy | 5 | 2.6 | 4.2 |
| CC(1/2) | 0.989 | 0.918 | 0.823 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 8 mg/mL protein 23% PEG Smear broad, 0.1 Na/K tartrate, 12 % Ethylenglycol, cacodylate pH 5.5 |
