7QFY
Fusarium oxysporum M36 protease without the propeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 41 |
| Unit cell lengths | 57.371, 57.371, 105.895 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.570 - 1.620 |
| R-factor | 0.1494 |
| Rwork | 0.148 |
| R-free | 0.17310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k90 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.921 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.444 | 1.646 |
| High resolution limit [Å] | 1.618 | 1.618 |
| Number of reflections | 42678 | 2196 |
| <I/σ(I)> | 14.2 | |
| Completeness [%] | 97.9 | |
| Redundancy | 13.6 | |
| CC(1/2) | 1.000 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES pH 7.5, 25% Polyethylene glycol 3,350 |
