7QFL
Crystal structure of S-layer protein SlpA from Lactobacillus acidophilus, domain II (aa 199-308)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-01 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0332 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 55.851, 55.851, 63.732 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.368 - 1.400 |
Rwork | 0.170 |
R-free | 0.19810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7qec |
RMSD bond length | 0.016 |
RMSD bond angle | 2.032 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.370 | 1.420 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.085 | 0.850 |
Rmeas | 0.095 | 0.953 |
Rpim | 0.042 | 0.427 |
Number of reflections | 23163 | 21345 |
<I/σ(I)> | 13.6 | |
Completeness [%] | 100.0 | |
Redundancy | 9.5 | 9.1 |
CC(1/2) | 0.997 | 0.821 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | protein stock solution of 18.5 mg/mL in 20 mM Hepes pH 8 and 100 mM NaCl; JCSG+ screen condition 1-1 (0.18 M lithium sulfate, 90 mM sodium acetate pH 4.5 and 45 % w/v PEG 400) with protein end concentration of 12.3 mg/mL corresponding to 66.67 % of protein solution in the 1.5 uL drop. |