7QFI

Crystal structure of S-layer protein SlpX from Lactobacillus acidophilus, domain I (aa 31-182)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-26
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.87313
Spacegroup name	P 1 21 1
Unit cell lengths	37.350, 38.347, 82.745
Unit cell angles	90.00, 93.93, 90.00

Refinement procedure

Resolution	41.280 - 1.500
Rwork	0.187
R-free	0.22240
Structure solution method	AB INITIO PHASING
RMSD bond length	0.013
RMSD bond angle	1.726
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	Arcimboldo
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.280	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.073	0.479
Rmeas	0.101	0.653
Rpim	0.069	0.441
Number of reflections	37359	3658
<I/σ(I)>	7.7
Completeness [%]	99.1
Redundancy	3.2	2.9
CC(1/2)	0.995	0.755

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	Protein stock solution of 15 mg/mL in 20 mM Hepes pH 8 and 100 mM NaCl; SG+ screen condition 50 (25 % w/v PEG 3350) with protein end concentration of 7.5 mg/mL corresponding to 50% of protein solution in the 1.0 uL drop