7QF8

Crystal structure of a bacterial pyranose 2-oxidase from Pseudoarthrobacter siccitolerans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-26
Detector	DECTRIS PILATUS3 X 2M
Wavelength(s)	0.8731
Spacegroup name	C 2 2 21
Unit cell lengths	78.448, 78.796, 151.313
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.400 - 2.009
R-factor	0.1992
Rwork	0.199
R-free	0.24420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mif
RMSD bond length	0.008
RMSD bond angle	0.940
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MoRDa
Refinement software	BUSTER (2.10.3 (18-SEP-2020))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.400	2.100
High resolution limit [Å]	2.000	2.000
Number of reflections	31572	5031
<I/σ(I)>	9.57
Completeness [%]	99.8
Redundancy	11.4
CC(1/2)	0.996	0.653

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293.15	2 M ammonium sulfate and 0.1 M Tris-HCl, pH 8.5