7QF4

Structure of the R57Q mutant of miniSOG expressed in E. coli in LB medium enriched with riboflavin

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.984
Spacegroup name	P 43 21 2
Unit cell lengths	39.930, 39.930, 134.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.282 - 1.170
Rwork	0.146
R-free	0.18210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6gpu
RMSD bond length	0.016
RMSD bond angle	2.167
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.290	1.200
High resolution limit [Å]	1.170	1.170
Rmeas	0.060	1.000
Number of reflections	37774	2702
<I/σ(I)>	17.9	2
Completeness [%]	99.7	99.1
Redundancy	8.1
CC(1/2)	0.999	0.646

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	100 mM Tris-Hcl pH 8.0, 20 mM MgCl2, 28% PEG 4000, 15 mM CoCl2