7QF2

Structure of miniSOG reconstituted with riboflavin as a cofactor

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.973
Spacegroup name	P 43 21 2
Unit cell lengths	39.900, 39.900, 134.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.243 - 1.070
Rwork	0.130
R-free	0.15530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6gpu
RMSD bond length	0.019
RMSD bond angle	2.200
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.250	1.100
High resolution limit [Å]	1.070	1.070
Rmeas	0.090	1.120
Number of reflections	91259	6739
<I/σ(I)>	11.5	1.12
Completeness [%]	100.0	99.9
Redundancy	6.5	5.6
CC(1/2)	0.990	0.563

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	100 mM Tris-HCl pH 8.0, 20 mM MgCl2, 28% PEG 4000, 15 mM CoCl2