7QF1

Crystal structure of the SARS-CoV-2 RBD in complex with the human antibody CV2.6264

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-07-24
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 21 21 21
Unit cell lengths	67.163, 191.319, 220.928
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.210 - 2.800
R-factor	0.2299
Rwork	0.229
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m0j 5vag
Data reduction software	XDS
Data scaling software	Aimless (0.2.7)
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.210	49.210	2.860
High resolution limit [Å]	2.800	13.430	2.800
Rmerge	0.445	0.047	6.770
Rmeas	0.453	0.048	6.894
Rpim	0.086	0.010	1.298
Total number of observations	1950768	15788	125885
Number of reflections	71215	742	4499
<I/σ(I)>	10.4	49.9	0.7
Completeness [%]	100.0	98.2	100
Redundancy	27.4	21.3	28
CC(1/2)	0.996	1.000	0.424

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296	0.1 M NaAc, 7% PEG 6000, 30% ethanol