7QEU
human Connexin 26 at 55mmHg PCO2, pH7.4: two masked subunits, class B
EM specimen
Vitrification | Yes |
Instrument | LEICA EM GP |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Energy Filter | GIF Bioquantum |
Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 2.7 |
Number of Particle | 1679668 |
Software | RELION,EPU,Gctf,Coot,PHENIX |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |