7QEH

LTA-binding domain of SlpA, the S-layer protein from Lactobacillus amylovorus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-01
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9789
Spacegroup name	P 21 21 21
Unit cell lengths	30.561, 43.481, 89.107
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.080 - 1.670
R-factor	0.1655
Rwork	0.163
R-free	0.19040
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	0.998
Data reduction software	XDS
Phasing software	SHELXCD
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.080	1.734
High resolution limit [Å]	1.670	1.670
Rmerge	0.109	0.860
Rmeas	0.114
Rpim	0.032	0.316
Number of reflections	26186	1361
<I/σ(I)>	19.84	3.2
Completeness [%]	99.4
Redundancy	12.2
CC(1/2)	0.999	0.805

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Morpheus screen condition #2-29 (20 mM of each carboxylic acid (sodium formate, ammonium acetate, sodium citrate tribasic dehydrate, sodium potassium tartrate tetrahydrate, and sodium oxamate), 0.1 M sodium HEPES- MOPS pH 7.5 with 20 % v/v PEG 500 MME and 10 % w/v PEG 20000). A protein stock solution of 20 mg/mL in 10 mM HEPES pH 7 and 100 mM NaCl was used. 1uL drop with 1:1 ration protein to reservoir solution.