7QDI
Structure of octameric left-handed 310-helix bundle: D-310HD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-05 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9204 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 33.238, 44.976, 194.192 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.590 - 2.340 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.23940 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.835 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.590 | 2.425 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.114 | 1.062 |
| Rmeas | 0.120 | |
| Rpim | 0.036 | 0.327 |
| Number of reflections | 12818 | 1056 |
| <I/σ(I)> | 11.97 | 1.98 |
| Completeness [%] | 97.1 | 83.68 |
| Redundancy | 10.9 | 11.3 |
| CC(1/2) | 0.999 | 0.824 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 0.1M (Sodium malonate dibasic monohydrate, Imidazole, Boric acid), 25% w/v PEG 1500 at pH 6.0 |
