7QD8
Cryo-EM structure of Tn4430 TnpA transposase from Tn3 family in apo state
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K2 QUANTUM (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.6 |
| Number of Particle | 4751313 |
| Software | cryoSPARC,EPU,CTFFIND,Coot,PHENIX |
| Symmetry | C2 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
