7QD8
Cryo-EM structure of Tn4430 TnpA transposase from Tn3 family in apo state
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Energy Filter | GIF Bioquantum |
Detector | GATAN K2 QUANTUM (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.6 |
Number of Particle | 4751313 |
Software | cryoSPARC,EPU,CTFFIND,Coot,PHENIX |
Symmetry | C2 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |