7QD2
Structure of the orange carotenoid protein from Planktothrix agardhii binding canthaxanthin in the P21 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9998 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.460, 65.940, 77.280 |
| Unit cell angles | 90.00, 102.26, 90.00 |
Refinement procedure
| Resolution | 43.600 - 1.400 |
| R-factor | 0.2019 |
| Rwork | 0.201 |
| R-free | 0.23020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mg1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.949 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19_4080) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.600 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.036 | 0.394 |
| Rmeas | 0.046 | 0.507 |
| Rpim | 0.028 | 0.315 |
| Number of reflections | 115206 | 11285 |
| <I/σ(I)> | 11.47 | 1.9 |
| Completeness [%] | 93.9 | 92.54 |
| Redundancy | 2.4 | 2.4 |
| CC(1/2) | 0.998 | 0.852 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.1 M sodium acetate, pH 5 and 10-20% PEG4000 |
