7QD1
Structure of the orange carotenoid protein from Planktothrix agardhii binding echinenone in the P21 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.363, 65.902, 77.372 |
Unit cell angles | 90.00, 102.35, 90.00 |
Refinement procedure
Resolution | 41.710 - 1.710 |
R-factor | 0.1824 |
Rwork | 0.181 |
R-free | 0.21590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mg1 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.340 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (1.19_4080) |
Refinement software | PHENIX (1.19_4080) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.580 | 1.750 |
High resolution limit [Å] | 1.710 | 1.710 |
Rmerge | 0.063 | 0.685 |
Rmeas | 0.074 | 0.877 |
Rpim | 0.038 | 0.547 |
Number of reflections | 60669 | 2735 |
<I/σ(I)> | 11.16 | 1.21 |
Completeness [%] | 90.0 | 54.8 |
Redundancy | 3.5 | 2.4 |
CC(1/2) | 0.997 | 0.426 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 0.2 M Bis-Tris pH 5.5, 20%-25% PEG 3550 |