7QD0
Structure of the orange carotenoid protein from Planktothrix agardhii binding echinenone in the C2 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9537 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 81.400, 64.870, 61.690 |
Unit cell angles | 90.00, 117.91, 90.00 |
Refinement procedure
Resolution | 32.440 - 1.700 |
R-factor | 0.1798 |
Rwork | 0.178 |
R-free | 0.22280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mg1 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.340 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (1.19_4080) |
Refinement software | PHENIX (1.19_4080) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.440 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.046 | 0.723 |
Rmeas | 0.055 | 0.723 |
Rpim | 0.030 | 0.388 |
Number of reflections | 30918 | 3107 |
<I/σ(I)> | 13.78 | 1.85 |
Completeness [%] | 98.6 | 99.42 |
Redundancy | 3.3 | 3.4 |
CC(1/2) | 0.999 | 0.843 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.1 M sodium acetate, pH 5 and 10-20% PEG4000 |