7QCZ
Structure of the orange carotenoid protein from Planktothrix agardhii binding canthaxanthin in the C2 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2019-10-23 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 82.010, 67.500, 61.940 |
Unit cell angles | 90.00, 117.82, 90.00 |
Refinement procedure
Resolution | 18.260 - 1.850 |
R-factor | 0.1787 |
Rwork | 0.175 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mg1 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.854 |
Data reduction software | TRUNCATE |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.19_4080) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.230 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.052 | 0.581 |
Rmeas | 0.066 | 0.739 |
Rpim | 0.040 | 0.451 |
Number of reflections | 23806 | 2022 |
<I/σ(I)> | 11.96 | 1.83 |
Completeness [%] | 92.9 | 79.92 |
Redundancy | 2.4 | 2.3 |
CC(1/2) | 0.997 | 0.675 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 0.2 M Bis-Tris pH 5.5, 20%-25% PEG 3550 |