7QCE
Crystal structure of an atypical PHD finger of VIN3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-06 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 58.641, 58.641, 225.395 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.840 - 2.100 |
| R-factor | 0.23198 |
| Rwork | 0.230 |
| R-free | 0.26450 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.286 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.840 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.135 | |
| Number of reflections | 27349 | 2183 |
| <I/σ(I)> | 13.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 19.6 | |
| CC(1/2) | 0.997 | 0.810 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 0.06M Morpheus Divalents, 1.1M Morpheus Buffer System pH 6.5, 10% PEG 20K, 20% PEG 500 MME |
