7QC8
HisF-C9A-D11E-V33A_L50H_I52H mutant in complex with Zn(II) from T. maritima
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 96.680, 96.680, 154.420 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 1.800 |
R-factor | 0.1393 |
Rwork | 0.138 |
R-free | 0.15920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7qc3 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.275 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.066 | 0.596 |
Number of reflections | 39147 | 5862 |
<I/σ(I)> | 25.3 | 4.4 |
Completeness [%] | 97.4 | 99.4 |
Redundancy | 12.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 292 | 100 mM TRIS, 23% PEG 3350 |