7QC0
Crystal structure of Cadmium translocating P-type ATPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-12 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 89.028, 94.532, 128.774 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.270 - 3.110 |
| R-factor | 0.2459 |
| Rwork | 0.245 |
| R-free | 0.26830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7qbz |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.767 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.270 | 3.220 |
| High resolution limit [Å] | 3.110 | 3.110 |
| Number of reflections | 20134 | 1963 |
| <I/σ(I)> | 17.8 | 1.12 |
| Completeness [%] | 97.4 | 99.8 |
| Redundancy | 13.3 | |
| CC(1/2) | 1.000 | 0.475 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292.15 | 200 mM magnesium formate, 14 % (v/v) PEG5000, 100 mM sodium acetate, pH=4.0, and 0.5 % (v/v) 2-propanol was added as an additive. |
