7QBP
Crystal structure of R2-like ligand-binding oxidase from Saccharopolyspora Erythraea
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-06-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 103.173, 103.173, 61.574 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.050 - 1.380 |
R-factor | 0.1522 |
Rwork | 0.151 |
R-free | 0.16540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ee4 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.383 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.050 | 1.429 |
High resolution limit [Å] | 1.380 | 1.380 |
Rmerge | 0.063 | 1.782 |
Rmeas | 0.064 | 1.818 |
Number of reflections | 68755 | 6772 |
<I/σ(I)> | 29.88 | 1.79 |
Completeness [%] | 100.0 | 100 |
Redundancy | 26.1 | |
CC(1/2) | 1.000 | 0.755 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 294 | 200 mM Lithium sulfate, 100 mM Sodium acetate pH 4.6, and 50 % (w/v) PEG 400 |