7QBF

TC:CD320 in complex with nanobody TC-Nb34

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	104.822, 104.822, 167.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.270 - 1.850
R-factor	0.1759
Rwork	0.174
R-free	0.20480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zrp
RMSD bond length	0.012
RMSD bond angle	2.577
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.270	1.920
High resolution limit [Å]	1.850	1.850
Rmerge	0.027
Number of reflections	158598	15319
<I/σ(I)>	1.89
Completeness [%]	98.2
Redundancy	2
CC(1/2)	0.999	0.442

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	200 mM Ammonium Iodide, 16% w/v PEG 3350 and 1.4 x 105-fold diluted seed stocks from crystals of the same protein, grown in similar conditions