7QAC

The T2 structure of polycrystalline cubic human insulin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID22
Synchrotron site	ESRF
Beamline	ID22
Temperature [K]	293
Detector technology	SCINTILLATION
Collection date	2015-12-08
Detector	Cyberstar LaBr3
Wavelength(s)	1.299740
Spacegroup name	I 21 3
Unit cell lengths	78.860, 78.860, 78.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.000 - 2.290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	9ins
RMSD bond length	0.015
RMSD bond angle	1.430
Data reduction software	PRODD
Phasing software	MOLREP
Refinement software	GSAS

Data quality characteristics

	Overall
Low resolution limit [Å]	39.430
High resolution limit [Å]	2.288
Number of reflections	3816

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	8.56	293	13.494 mg/ml insulin, 0.818 mM zinc acetate, 10.563 mM sodium thiocyanate, 0.4 M sodium potassium phosphate (Na2HPO4/KH2PO4)
2	BATCH MODE	7.88	293	13.494 mg/ml insulin, 0.818 mM zinc acetate, 10.563 mM sodium thiocyanate, 0.4 M sodium potassium phosphate (Na2HPO4/KH2PO4)
3	BATCH MODE	8.02	293	13.494 mg/ml insulin, 0.818 mM zinc acetate, 10.563 mM sodium thiocyanate, 0.4 M sodium potassium phosphate (Na2HPO4/KH2PO4)
4	BATCH MODE	8.17	293	13.494 mg/ml insulin, 0.818 mM zinc acetate, 10.563 mM sodium thiocyanate, 0.4 M sodium potassium phosphate (Na2HPO4/KH2PO4)
5	BATCH MODE	8.26	293	13.494 mg/ml insulin, 0.818 mM zinc acetate, 10.563 mM sodium thiocyanate, 0.4 M sodium potassium phosphate (Na2HPO4/KH2PO4)
6	BATCH MODE	8.17	293	13.494 mg/ml insulin, 0.818 mM zinc acetate, 10.563 mM sodium thiocyanate, 0.4 M sodium potassium phosphate (Na2HPO4/KH2PO4)