7QA4
Crystal structure of stabilized H3N2 A/Hong Kong/1/1968 Hemagglutinin at 2.2 Angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-01-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.999924 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 154.094, 154.094, 154.094 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 108.960 - 2.190 |
| R-factor | 0.201 |
| Rwork | 0.198 |
| R-free | 0.25060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | none |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.411 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 108.960 | 108.960 |
| High resolution limit [Å] | 2.192 | 2.192 |
| Rmerge | 0.085 | 2.977 |
| Number of reflections | 31315 | 31315 |
| <I/σ(I)> | 24.9 | 1.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 25.6 | 26.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 20.00 %w/v PEG 3350, 0.2 M LiNO3 |
