7Q8C
Leishmania major actin filament in ADP-state
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 2.72 |
| Software | RELION,UCSF ChimeraX,ISOLDE,PHENIX,CTFFIND,UCSF Chimera,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
